One strategy to overcome filariasis cases is by turning off vectors, namely Culex sp. Larvae, and usually using synthetic larvacides. The long-term use of chemicals as larvacides can cause resistance to larvae and environmental pollution. Tobacco leaves (Nicotiana tabacum L) can cause death in larvae because they contain nicotine and essential oils, and are safe for animals and for the environment. This study aims to determine the potential of tobacco leaf extract (Nicotiana tabacum L) as biolarvasida.
The research method is experimental, with the research design used is post test only control group design. Samples in the form of Culex sp instar III mosquito larvae. The study used 5 treatments and 5 repetitions with 20 larvae tested for each treatment. The concentration of tobacco leaves (Nicotiana tabacum L) is 0% (control); 0.003%; 0.006%; 0.012%; 0.025%. Data analysis was performed analytically using Probit test and different test (One Way Anova).
The results showed that tobacco leaf extract (Nicotiana tabacum L.) had the potential to be biolarvasida and had a mortality rate of 0% concentration (control); 0.003%; 0.006%; 0.012% and 0.025% by 0%; 40%; 76%; 99.5% and 100%. The probit test shows the LC98 concentration of 0.022%. Different test showed that there was a significant difference between the control group and tobacco leaf extract (Nicotiana tabacum L.) on the death of Culex sp mosquito larvae (α <0.05).
Suggestions that can be given is that tobacco leaves (Nicotiana tabacum L) can be used as biolarvasida and used as an alternative in addition to the use of chemical larvacides and further research is needed on the Lethal Time needed to kill Culex sp. Larvae.
Keywords: Biolarvasida, tobacco leaf extract (Nicotiana tabacum L.), Culex sp