Uji UJI AKTIVITAS ANTIOKSIDAN NANOPARTIKEL KITOSAN DARI LIMBAH KULIT UDANG (Litopenaeus vannamei) MENGGUNAKAN METODE DPPH (2,2-diphenyl-1-picrylhydrazyl)
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How to Cite

Lukiyono, Y. (2021). Uji UJI AKTIVITAS ANTIOKSIDAN NANOPARTIKEL KITOSAN DARI LIMBAH KULIT UDANG (Litopenaeus vannamei) MENGGUNAKAN METODE DPPH (2,2-diphenyl-1-picrylhydrazyl). Prosiding Seminar Nasional Kesehatan Poltekkes Kemenkes Surabaya 2020, 2(1). Retrieved from http://semnas.poltekkesdepkes-sby.ac.id/index.php/2020/article/view/313

Abstract

ABSTRACT-Antioxidants are compounds that have an important role in maintain health because it can prevent free radical, thus inhibit oxidation reactions in the body causes various degenerative diseases. This study aims to analyze utilization of chitosan nanoparticles derived from waste shell shrimp (Litopenaus vannamei) as an antioxidant. Antioxidant activity qualitatively tested with Thin Layer Chomatograpgy (TLC) autograph. Result obtained from Chitosan nanoparticles produce clear stain with violet background. Then  quantitative testing used DPPH (2,2-diphenyl-1-picrylhydrazyl) method with spektrofotometer UV-Visible by calculating the free radical inhibition percentage by chitosan nanoparticles at a wavelength of  516,5 nm  by using vitamin C as comparison with IC50 value of 2,4703 ppm. Chitosan nanoparticles hasn’t an antioxidant activity with IC50 value of 2731,9 ppm..

Keywords: Chitosan Nanoparticles, Antioxidant, TLC Autograph, DPPH, IC50.

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